6HCZ
Sjoegren syndrome/scleroderma autoantigen 1 (SSSCA1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.239530 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 49.490, 49.490, 164.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.22354 |
| Rwork | 0.222 |
| R-free | 0.26030 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.551 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.390 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | |
| Rmeas | 0.055 | |
| Rpim | 0.018 | 0.631 |
| Number of reflections | 9709 | 701 |
| <I/σ(I)> | 26.2 | 1.4 |
| Completeness [%] | 99.3 | 98.5 |
| Redundancy | 9.2 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 3.5 M sodium formate pH 7.0, 10% glycerol |
