6HCG
Klebsiella pneumoniae type II secretion system outer membrane complex. PulD, PulS and PulC HR domain.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.3 |
| Software | RELION,Gctf |
| Symmetry | C15 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
