6HC7

The crystal structure of BSAP, a zinc aminopeptidase from Bacillus subtilis (medium resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.282
Spacegroup name	P 63
Unit cell lengths	225.833, 225.833, 42.654
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	73.921 - 2.500
R-factor	0.1814
Rwork	0.178
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cp7
RMSD bond length	0.009
RMSD bond angle	1.172
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	COMO
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.053	0.250
Number of reflections	43610
<I/σ(I)>	13.1
Completeness [%]	98.8	86.3
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	20% PEG 2K MME, 0.1M LiSO4, 4mM MnCl2, 0.1M acetate pH 5.6