6HC5

The structure of the G. violaceus guanidine II riboswitch P2 stem-loop with audouine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9196
Spacegroup name	H 3 2
Unit cell lengths	55.826, 55.826, 133.363
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.913 - 1.413
R-factor	0.1765
Rwork	0.175
R-free	0.19890
RMSD bond length	0.012
RMSD bond angle	1.801
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3228: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.913	1.437
High resolution limit [Å]	1.413	1.413
Rmerge	0.047	1.920
Rpim	0.021	0.813
Number of reflections	28892	775
<I/σ(I)>	18.1
Completeness [%]	98.0	100
Redundancy	6.3	6.5
CC(1/2)	1.000	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.01 M Magnesium Acetate, 0.05 M MES pH 5.0, 2.5 M Ammonium Sulfate