6HC1
Bdellovibrio bacteriovorus DgcB FHA in complex with phosphorylated N-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 68.750, 68.750, 191.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.540 - 1.490 |
| R-factor | 0.1437 |
| Rwork | 0.142 |
| R-free | 0.17750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.698 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.540 | 56.840 | 1.530 |
| High resolution limit [Å] | 1.490 | 6.660 | 1.490 |
| Rmerge | 0.130 | 0.065 | 2.796 |
| Rmeas | 0.132 | 0.066 | 2.856 |
| Rpim | 0.022 | 0.012 | 0.578 |
| Total number of observations | 18340 | 78317 | |
| Number of reflections | 44707 | 644 | 3238 |
| <I/σ(I)> | 16.1 | 45.1 | 1.2 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 33.5 | 28.5 | 24.2 |
| CC(1/2) | 0.999 | 0.999 | 0.545 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1M sodium hepes pH 7, 20% PEG8000 |
