6HC0

Bdellovibrio bacteriovorus DgcB FHA domain, tail complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	69.350, 69.370, 128.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.460 - 1.870
R-factor	0.178
Rwork	0.177
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.790
Data reduction software	XDS
Data scaling software	Aimless (0.5.29)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.460	36.530	1.920
High resolution limit [Å]	1.870	8.360	1.870
Rmerge	0.081	0.033	1.922
Rmeas	0.085	0.035	2.003
Rpim	0.023	0.010	0.558
Number of reflections	52209	682	3787
<I/σ(I)>	19.1
Completeness [%]	100.0	99.1	100
Redundancy	12.8	10.8	12.6
CC(1/2)	1.000	0.999	0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	291	0.2M ammonium formate, 10% polyvinylpyrrolidone, 20% PEG 8000