6HBX
The structure of the G. violaceus guanidine II riboswitch P2 stem-loop with ethylguanidine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-05 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9197 |
Spacegroup name | H 3 2 |
Unit cell lengths | 56.070, 56.070, 134.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.035 - 1.540 |
R-factor | 0.1917 |
Rwork | 0.190 |
R-free | 0.23000 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.380 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3228: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.035 | 1.570 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.053 | 1.436 |
Rpim | 0.026 | 0.696 |
Number of reflections | 22399 | 584 |
<I/σ(I)> | 17.3 | |
Completeness [%] | 97.7 | 96.5 |
Redundancy | 4.9 | 5.1 |
CC(1/2) | 1.000 | 0.484 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.01 M Magnesium Acetate, 0.05 M MES pH 5.6, 2.5 M Ammonium Sulfate |