6HBD
Crystal structure of MSMEG_1712 from Mycobacterium smegmatis in complex with Beta-D-Galactofuranose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 118.300, 118.300, 231.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 105.310 - 2.440 |
R-factor | 0.229 |
Rwork | 0.227 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hb0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.310 | 2.767 |
High resolution limit [Å] | 2.440 | 2.445 |
Number of reflections | 118103 | |
<I/σ(I)> | 20.2 | |
Completeness [%] | 94.7 | |
Redundancy | 27.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 8.0 21 % PEG 400 0.325 M NaOAc 0.1 M NaCl |