6HBB
Crystal Structure of the small subunit-like domain 1 of CcmM from Synechococcus elongatus (strain PCC 7942)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-21 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96600 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.302, 87.821, 36.467 |
| Unit cell angles | 90.00, 107.44, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.200 |
| R-factor | 0.1609 |
| Rwork | 0.159 |
| R-free | 0.19520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hba |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.132 |
| Data reduction software | XDS (VERSION November 3, 2014) |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | MOLREP (11.0.05) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.910 | 43.910 | 1.220 |
| High resolution limit [Å] | 1.200 | 6.350 | 1.200 |
| Rmerge | 0.060 | 0.045 | 0.447 |
| Rmeas | 0.072 | 0.055 | 0.578 |
| Rpim | 0.041 | 0.031 | 0.361 |
| Total number of observations | 140389 | ||
| Number of reflections | 48992 | 355 | 1728 |
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 96.0 | 98.2 | 64.6 |
| Redundancy | 2.9 | 3.2 | 2 |
| CC(1/2) | 0.997 | 0.995 | 0.723 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 277 | 1.95 M ammonium sulfate and 0.1 M Na-acetate pH 4.5 |
