6HB9

Crystal structure of the GABARAP in complex with the UBA5 LIR motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-08-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	33.776, 58.010, 36.421
Unit cell angles	90.00, 99.35, 90.00

Refinement procedure

Resolution	33.327 - 1.300
R-factor	0.1911
Rwork	0.190
R-free	0.21320
RMSD bond length	0.008
RMSD bond angle	1.111
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.940	1.370
High resolution limit [Å]	1.300	1.300
Number of reflections	32955
<I/σ(I)>	20.9
Completeness [%]	96.7
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6 M sodium phosphate, 0.1 M HEPES, pH7.5