6HAU
KSHV PAN RNA Mta-response element fragment complexed with the globular domain of herpesvirus saimiri ORF57
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9282 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.657, 105.261, 118.554 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 78.710 - 1.860 |
R-factor | 0.16 |
Rwork | 0.158 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Apo HVS ORF57 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.924 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.710 | 1.925 |
High resolution limit [Å] | 1.858 | 1.858 |
Rmerge | 0.073 | 0.725 |
Rmeas | 0.079 | 0.777 |
Number of reflections | 62577 | 6135 |
<I/σ(I)> | 18.23 | 2.67 |
Completeness [%] | 99.9 | 99.79 |
Redundancy | 7.5 | 7.7 |
CC(1/2) | 0.999 | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.06 M divalents (0.3M Magnesium chloride hexahydrate; 0.3M Calcium chloride dehydrate), 0.1 M (Imidazole, MES) Buffer System pH 6.5, 50 % v/v GOL_P4K (Morpheus HT96 A3 Molecular Dimensions) |