6HAT
Globular domain of herpesvirus saimiri ORF57
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.108, 118.652, 130.702 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.466 - 1.856 |
R-factor | 0.1497 |
Rwork | 0.149 |
R-free | 0.16800 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.162 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.470 | 1.923 |
High resolution limit [Å] | 1.856 | 1.856 |
Rmerge | 0.083 | 0.967 |
Rmeas | 0.087 | 1.005 |
Number of reflections | 77002 | 7497 |
<I/σ(I)> | 18.04 | 2.55 |
Completeness [%] | 99.8 | 98.48 |
Redundancy | 13.3 | 13.5 |
CC(1/2) | 0.999 | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.12 M Alcohols (0.2 M 1,6-Hexanediol; 0.2 M 1-Butanol; 0.2 M 1,2-Propanediol; 0.2M 2-Propanol; 0.2 M 1,4-Butanediol; 0.2M 1,3-Propanediol), 0.1 M (Imidazole, MES) Buffer System pH 6.5, 50 % v/v GOL_P4K Mix (Morpheus HT96 D3 Molecular Dimensions) |