6HAO
MamM CTD H264E - Cadmium form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 53.661, 53.661, 58.074 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.470 - 2.400 |
R-factor | 0.24499 |
Rwork | 0.238 |
R-free | 0.31440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w5x |
RMSD bond length | 0.005 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0216) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.470 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.169 | |
Number of reflections | 3988 | |
<I/σ(I)> | 11.9 | 2.3 |
Completeness [%] | 98.6 | 88.4 |
Redundancy | 9.2 | 4.7 |
CC(1/2) | 0.994 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M MgCl2, 0.1M BIS-TRIS pH=6.5, 25% PEG 3350, 1.7mM CdCl2 |