6HAM
Adenylate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 43 |
| Unit cell lengths | 58.440, 58.440, 77.837 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.440 - 2.550 |
| R-factor | 0.20511 |
| Rwork | 0.203 |
| R-free | 0.24325 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.669 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.730 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 8598 | 864 |
| <I/σ(I)> | 23.43 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 14% PEG 4000 0.05M Tris pH=8 |
