6HAI

AlbAM131A mutant in complex with albicidin , albicidin resistance protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.87313
Spacegroup name	C 2 2 21
Unit cell lengths	53.260, 123.497, 161.171
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.014 - 2.200
R-factor	0.2121
Rwork	0.210
R-free	0.23990
RMSD bond length	0.007
RMSD bond angle	0.526
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.010	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.047	0.339
Number of reflections	27360
<I/σ(I)>	7.79
Completeness [%]	99.5
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2-0.4 M Ammonium Sulfate, 0.8-1.2 M Lithium Sulfate, 0.1 M sodium citrate tribasic