6HA5
AFGH61B L90V/D131S/M134L/A141W VARIANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.03982 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.100, 44.280, 60.000 |
| Unit cell angles | 89.64, 108.32, 108.95 |
Refinement procedure
| Resolution | 19.370 - 1.870 |
| R-factor | 0.1411 |
| Rwork | 0.139 |
| R-free | 0.18150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zud |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.313 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 27929 | |
| <I/σ(I)> | 17.8 | 6.4 |
| Completeness [%] | 96.4 | 95 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | reservoir consisting of 0.1 M cobalt chloride, 0.1 M Tris pH 8.5; 20 %w/v Polyvinylpyrrolidone K15 and a 1:1 protein reservoir ratio in 0.3 microliter drop |
