6HA2
MamM CTD D249H-H285D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 35.737, 94.302, 52.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.150 - 1.500 |
| R-factor | 0.16256 |
| Rwork | 0.161 |
| R-free | 0.19872 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.603 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0216) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.150 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.046 | |
| Number of reflections | 14775 | |
| <I/σ(I)> | 18.6 | 1.4 |
| Completeness [%] | 99.7 | 94.1 |
| Redundancy | 7.8 | 7.3 |
| CC(1/2) | 1.000 | 0.582 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M NaAcetate pH=4.5, 25% PEG 3350, 1.7mM MnCl2 |
