6H9O

Complex of the periplasmic domains of bacterial cell division proteins FtsQ and FtsB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97950
Spacegroup name	C 1 2 1
Unit cell lengths	117.221, 48.011, 131.619
Unit cell angles	90.00, 112.40, 90.00

Refinement procedure

Resolution	29.834 - 2.800
R-factor	0.2322
Rwork	0.230
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vh1
RMSD bond length	0.012
RMSD bond angle	1.637
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.077	0.744
Rpim	0.051	0.488
Number of reflections	15922	2374
<I/σ(I)>	10.6	1.8
Completeness [%]	98.5	99.7
Redundancy	3.2	3.3
CC(1/2)	0.998	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.057 M potassium thiocyanate, 10 % PEG 550 MME, 0.1 M sodium cacodylate pH 6.5.