6H9L

Kcr_0859 delta TM from Korarchaeum cryptofilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97895
Spacegroup name	P 21 21 2
Unit cell lengths	197.520, 48.880, 51.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.300 - 2.190
R-factor	0.24417
Rwork	0.243
R-free	0.27331
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.036
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.300	2.320
High resolution limit [Å]	2.190	2.190
Rmerge	0.086	0.585
Number of reflections	26105
<I/σ(I)>	9.23	1.74
Completeness [%]	99.0	95.7
Redundancy	3.4	3.27
CC(1/2)	0.998	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	100 mM sodium cacodylate pH 6.5, 30% MPD, 5% PEG 2000