6H9J
Factor Inhibiting HIF (FIH) in complex with zinc, NOG and TRPV3 (229-255)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.400, 86.400, 149.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.635 - 1.830 |
R-factor | 0.1668 |
Rwork | 0.166 |
R-free | 0.18680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h2k |
RMSD bond length | 0.013 |
RMSD bond angle | 1.065 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.635 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.105 | 2.854 |
Rmeas | 0.108 | 2.928 |
Rpim | 0.025 | 0.649 |
Number of reflections | 50768 | 2483 |
<I/σ(I)> | 13.74 | 1.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.4 | 20.1 |
CC(1/2) | 0.998 | 0.497 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Hepes pH = 7.5, 1.8 M ammonium sulfate, 4.5% PEG400 |