6H9I
Csf5, CRISPR-Cas type IV Cas6 crRNA endonuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.966 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.850, 84.237, 71.472 |
Unit cell angles | 90.00, 100.35, 90.00 |
Refinement procedure
Resolution | 43.762 - 2.290 |
R-factor | 0.2247 |
Rwork | 0.223 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6h9h |
RMSD bond length | 0.004 |
RMSD bond angle | 0.792 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.762 | 2.370 |
High resolution limit [Å] | 2.288 | 2.290 |
Rmerge | 0.074 | |
Rmeas | 0.090 | |
Rpim | 0.050 | |
Number of reflections | 32620 | |
<I/σ(I)> | 10.83 | |
Completeness [%] | 98.3 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M di-sodium tartrate, 20% w/v PEG3350 |