6H9D
Muramidase domain of SpmX from Asticaccaulis excentricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2015-02-05 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.965000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 100.440, 100.440, 96.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.560 - 1.900 |
| R-factor | 0.21594 |
| Rwork | 0.211 |
| R-free | 0.25594 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2anx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.182 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.560 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 42611 | 6805 |
| <I/σ(I)> | 33.99 | 3.1 |
| Completeness [%] | 95.1 | 95 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 0.1 M Tris-HCl pH 8.5 0.2 M MgCl2 12% PEG 3350 |
