6H93
Beta-phosphoglucomutase from Lactococcus lactis with inorganic phosphate bound in an open conformer to 1.8 A.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.350, 117.140, 53.190 |
Unit cell angles | 90.00, 99.14, 90.00 |
Refinement procedure
Resolution | 32.050 - 1.770 |
R-factor | 0.18164 |
Rwork | 0.179 |
R-free | 0.23316 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2whe |
RMSD bond length | 0.012 |
RMSD bond angle | 1.494 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.050 | 1.800 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.085 | 1.426 |
Rmeas | 0.092 | 1.543 |
Rpim | 0.035 | 0.583 |
Number of reflections | 45050 | 2241 |
<I/σ(I)> | 12.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.8 | 6.9 |
CC(1/2) | 0.999 | 0.467 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 24-34% PEG 4000, 200 mM sodium acetate, 50 mM TRIS pH 7.5 |