6H91
Phosphorylated beta-phosphoglucomutase from Lactococcus lactis in an open conformer to 2.4 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97779 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.330, 117.230, 52.930 |
| Unit cell angles | 90.00, 97.75, 90.00 |
Refinement procedure
| Resolution | 58.620 - 2.380 |
| R-factor | 0.22299 |
| Rwork | 0.219 |
| R-free | 0.29875 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2whe |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.539 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.620 | 2.440 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.120 | 0.597 |
| Rmeas | 0.165 | 0.813 |
| Rpim | 0.084 | 0.438 |
| Number of reflections | 18430 | 1355 |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 99.0 | 98.9 |
| Redundancy | 3.7 | 3.3 |
| CC(1/2) | 0.987 | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 24-34% PEG 4000, 200 mM sodium acetate, 50 mM TRIS pH 7.5, The crystal was cryoprotected in its original mother liquor plus an additional 25% ethylene glycol and 30mM acetylphosphate. |
