6H8H

Molybdenum storage protein - in a recombinant and in vivo-like form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-11
Detector	PSI PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 63 2 2
Unit cell lengths	117.100, 117.100, 235.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.942 - 1.901
R-factor	0.2068
Rwork	0.206
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ndo
RMSD bond length	0.006
RMSD bond angle	2.508
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	141946
<I/σ(I)>	11.5
Completeness [%]	99.3
Redundancy	6.9
CC(1/2)	0.997	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M sodium citrate, 1 M NH4H2PO4, 10% glycerol