6H8F
Fragment of the C-terminal domain of the TssA component of the type VI secretion system from Burkholderia cenocepacia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.920000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 48.260, 62.700, 65.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.900 - 1.780 |
| R-factor | 0.19118 |
| Rwork | 0.189 |
| R-free | 0.23729 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.316 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.068 | 0.697 |
| Rpim | 0.039 | 0.387 |
| Number of reflections | 9502 | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 97.0 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M Citric acid pH 5.0, 20 % (w/v) PEG 6000 |
