6H8B
Molybdenum storage protein prepared under in vivo-like conditions and incubated with ATP and molybdate at 303 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-15 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 116.720, 116.720, 235.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.415 - 1.900 |
R-factor | 0.2343 |
Rwork | 0.234 |
R-free | 0.24680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ndo |
RMSD bond length | 0.019 |
RMSD bond angle | 2.994 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 74844 | |
<I/σ(I)> | 11.1 | 2 |
Completeness [%] | 99.5 | |
Redundancy | 4.7 | |
CC(1/2) | 0.998 | 0.837 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M sodium citrate, pH 5.6 1 M NH4H2PO4 10% glycerol |