6H8A
MamM CTD D249N - Cadmium form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.844, 94.661, 53.438 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.330 - 1.800 |
| R-factor | 0.1968 |
| Rwork | 0.192 |
| R-free | 0.23669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.592 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0216) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.145 | |
| Number of reflections | 9025 | 533 |
| <I/σ(I)> | 12.3 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.2 | 12.8 |
| CC(1/2) | 0.997 | 0.666 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Li2SO4, 0.1M BIS-TRIS pH=5.5, 25% PEG 3350, 1.7mM CdCl2 |
