6H88
MamM CTD D249N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.488, 62.826, 44.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.010 - 1.500 |
| R-factor | 0.18426 |
| Rwork | 0.182 |
| R-free | 0.22658 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.658 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.010 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.038 | |
| Number of reflections | 12949 | |
| <I/σ(I)> | 22.2 | 2.2 |
| Completeness [%] | 99.2 | 91 |
| Redundancy | 6.3 | 5.6 |
| CC(1/2) | 1.000 | 0.814 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES pH=7.5, 25% PEG 3350, 1.7mM MnCl2 |
