6H84
MamM CTD D249H - Cadmium form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977999 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.493, 94.516, 53.303 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.260 - 2.040 |
| R-factor | 0.19291 |
| Rwork | 0.190 |
| R-free | 0.22206 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.674 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0171) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.260 | 2.100 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.128 | |
| Number of reflections | 6119 | |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 99.7 | 95.6 |
| Redundancy | 12.9 | |
| CC(1/2) | 0.998 | 0.603 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M AmSu, 0.1M BIS-TRIS pH=5.5, 25% PEG 3350, 1.7mM CdCl2 |
