6H81
MamM CTD E289H - Nickel form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977999 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.060, 94.532, 53.539 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.270 - 1.500 |
| R-factor | 0.19853 |
| Rwork | 0.198 |
| R-free | 0.21308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.458 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0171) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.270 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.049 | |
| Number of reflections | 15422 | |
| <I/σ(I)> | 22.6 | 1.1 |
| Completeness [%] | 99.5 | 90.4 |
| Redundancy | 13.2 | |
| CC(1/2) | 0.999 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M AmSu, 0.1M BIS-TRIS pH=5.5, 25% PEG 3350, 1.7 mM NiCl2 |
