6H7Z
X-ray structure of human glutamate carboxypeptidase II (GCPII) in complex with a inhibitor RNA 2-65-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-21 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.34 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 101.222, 130.389, 158.469 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.17201 |
| Rwork | 0.170 |
| R-free | 0.20594 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3bi1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.790 |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.800 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.176 | 0.884 |
| Number of reflections | 70883 | 4537 |
| <I/σ(I)> | 11.9 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.2 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 34% (v/v) pentaerythritol propoxylate PO/OH 5/4, 2 % (w/v) PEG 3350, 100 mM Tris-HCl, pH 8.0 |
