6H7C
tRNA guanine transglycosylase H333A mutant apo structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.296, 65.013, 70.488 |
Unit cell angles | 90.00, 95.88, 90.00 |
Refinement procedure
Resolution | 26.469 - 1.681 |
R-factor | 0.161 |
Rwork | 0.160 |
R-free | 0.18440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pud |
RMSD bond length | 0.007 |
RMSD bond angle | 0.885 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.700 |
High resolution limit [Å] | 1.680 | 1.680 |
Number of reflections | 46188 | |
<I/σ(I)> | 12.96 | 3.5 |
Completeness [%] | 98.8 | 94.4 |
Redundancy | 3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100mM Tris, 10% DMSO, 9% PEG 8000 |