6H7C
tRNA guanine transglycosylase H333A mutant apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.296, 65.013, 70.488 |
| Unit cell angles | 90.00, 95.88, 90.00 |
Refinement procedure
| Resolution | 26.469 - 1.681 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.18440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pud |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.885 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.700 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Number of reflections | 46188 | |
| <I/σ(I)> | 12.96 | 3.5 |
| Completeness [%] | 98.8 | 94.4 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100mM Tris, 10% DMSO, 9% PEG 8000 |
