6H6P
UbiJ-SCP2 Ubiquinone synthesis protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-26 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.690, 94.550, 115.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.280 - 2.500 |
R-factor | 0.236 |
Rwork | 0.233 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6h6n |
RMSD bond length | 0.010 |
RMSD bond angle | 1.270 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.280 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 18749 | |
<I/σ(I)> | 9.13 | |
Completeness [%] | 99.9 | |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 225 mM Calcium Chloride, 100 mM MMT buffer pH 9, 11% w/v PEG 8000 |