6H6N
UbiJ-SCP2 Ubiquinone synthesis protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.6488 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.300, 67.930, 73.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.290 - 2.120 |
| R-factor | 0.198 |
| Rwork | 0.194 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.290 | 2.170 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Number of reflections | 13906 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 97.1 | |
| Redundancy | 22.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 225 mM Calcium Chloride, 100 mM MMT buffer pH 9, 11% w/v PEG 8000 |
