6H6D
Crystal structures of the murine class I major histocompatibility complex H-2Db in complex with adenovirus-derived peptide Ad10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-21 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.140, 69.240, 87.160 |
| Unit cell angles | 84.20, 86.69, 81.75 |
Refinement procedure
| Resolution | 19.980 - 2.400 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.23333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n5a |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.760 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.640 | |
| Number of reflections | 42622 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 90.0 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 1.8M ammonium sulphate, 0.1M Tris |
