6H6C
Carbomonoxy murine neuroglobin F106A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.940, 88.940, 110.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.150 - 1.750 |
| R-factor | 0.13501 |
| Rwork | 0.131 |
| R-free | 0.20892 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w92 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.879 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.150 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.093 | |
| Number of reflections | 16336 | |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.8 | |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.6 M ammonium sulfate, 0.1 M MES pH 6.5, 10% 1,4-dioxane |
