6H61
CryoEM structure of the MDA5-dsRNA filament with 89 degree twist and without nucleotide
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.02 |
| Number of Particle | 526596 |
| Software | RELION,EPU,Gctf,Coot,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
