6H5T
Intersectin SH3A short isoform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-19 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.895 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 86.792, 86.792, 141.768 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.692 - 1.689 |
| R-factor | 0.1763 |
| Rwork | 0.176 |
| R-free | 0.19080 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.245 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.689 | 1.689 |
| Rmeas | 0.083 | 0.960 |
| Number of reflections | 30662 | 4848 |
| <I/σ(I)> | 17 | 2.4 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 7.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25% (v/v) polyethylene glycol 550 mono-methyl ether, 100 mM MES-NaOH at pH 6.5, and 10 mM ZnSO4 |
