6H56
Effector domain of Pseudomonas aeruginosa VgrG2b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 31 |
Unit cell lengths | 78.310, 78.310, 115.344 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.930 - 3.200 |
R-factor | 0.2238 |
Rwork | 0.223 |
R-free | 0.23200 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.930 | 3.420 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.420 | |
Rmeas | 0.090 | 0.757 |
Number of reflections | 12896 | |
<I/σ(I)> | 10.9 | 2.3 |
Completeness [%] | 98.5 | 97.5 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M bis-tris, 45% polypropylene glycol P400 |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M bis-tris, 45% polypropylene glycol P400 |