6H4M
TarP-UDP-GlcNAc-3RboP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-14 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.00002 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.170, 210.750, 123.200 |
Unit cell angles | 90.00, 91.92, 90.00 |
Refinement procedure
Resolution | 48.440 - 2.730 |
R-factor | 0.19077 |
Rwork | 0.189 |
R-free | 0.23323 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.108 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 2.800 |
High resolution limit [Å] | 2.730 | 2.730 |
Number of reflections | 128233 | |
<I/σ(I)> | 8.4 | |
Completeness [%] | 99.9 | |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 25% PEG 3350, 250 mM magnesium chloride, 0.1 M sodium citrate, pH 5.7. |