6H4I
Usp28 catalytic domain apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-27 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 105.538, 200.518, 207.244 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.590 - 3.220 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | CRANK2 |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.590 | 3.493 |
| High resolution limit [Å] | 3.220 | 3.223 |
| Rpim | 0.041 | 0.562 |
| Number of reflections | 25908 | |
| <I/σ(I)> | 15.2 | 1.3 |
| Completeness [%] | 94.3 | 60.5 |
| Redundancy | 13.8 | |
| CC(1/2) | 0.999 | 0.417 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M citric acid pH 5.0 0.8 M Ammonium Sulfate |
