6H4C

A polyamorous repressor: deciphering the evolutionary strategy used by the phage-inducible chromosomal islands to spread in nature.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 3 2 1
Unit cell lengths	144.490, 144.490, 149.420
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	95.934 - 2.520
R-factor	0.1992
Rwork	0.197
R-free	0.24220
RMSD bond length	0.010
RMSD bond angle	1.210
Data reduction software	xia2
Data scaling software	Aimless (0.5.31)
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	95.934	64.150	2.590
High resolution limit [Å]	2.520	11.270	2.520
Rmerge	0.162	0.033	2.003
Rmeas	0.170	0.035	2.121
Rpim	0.052	0.012	0.696
Number of reflections	61269	775	4493
<I/σ(I)>	12
Completeness [%]	100.0	99.5	100
Redundancy	10.6	9.1	9.2
CC(1/2)	0.999	0.999	0.477

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	24% PEG 1500 20% Glicerol