6H4B
A polyamorous repressor: deciphering the evolutionary strategy used by the phage-inducible chromosomal islands to spread in nature.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | I 2 3 |
Unit cell lengths | 122.896, 122.896, 122.896 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.448 - 2.902 |
R-factor | 0.2587 |
Rwork | 0.254 |
R-free | 0.30520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Chain A 5mil chain B 6H48 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.495 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.900 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.099 | 1.038 |
Rpim | 0.183 | |
Number of reflections | 7002 | 1110 |
<I/σ(I)> | 23.7 | 4.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 33.3 | 33.4 |
CC(1/2) | 1.000 | 0.913 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294.15 | 10%PEG4000, 10%isopropanol, 0.1MNa-Citrate |