6H48

A polyamorous repressor: deciphering the evolutionary strategy used by the phage-inducible chromosomal islands to spread in nature.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97926
Spacegroup name	P 3 2 1
Unit cell lengths	77.357, 77.357, 37.318
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	66.990 - 2.200
R-factor	0.2491
Rwork	0.248
R-free	0.26390
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.002
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.993	38.679	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge		0.067	1.055
Rmeas	0.116	0.069	1.086
Rpim	0.027	0.017	0.253
Number of reflections	6522	243	843
<I/σ(I)>	14.4	7.1	0.7
Completeness [%]	96.7	99.6	88.3
Redundancy	18.4	16.6	16.7
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	40%PEG3350 0.1M Bis-Tris 0.2M Na-thiocyanate