6H3Z
Crystal structure of a C-terminal MIF4G domain in NOT1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03849 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.930, 79.770, 98.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.490 - 3.000 |
| R-factor | 0.1854 |
| Rwork | 0.182 |
| R-free | 0.24440 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 12140 | |
| <I/σ(I)> | 18.4 | 1.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 5.9 | |
| CC(1/2) | 1.000 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100 mM HEPES/NaOH pH 7.5, 1.0 M Ammonium Sulfate, 1.0 M Potassium Chloride |
