6H3T
Schmallenberg Virus Glycoprotein Gc Head Domain in Complex with scFv 1C11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980089 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 262.789, 44.397, 93.593 |
| Unit cell angles | 90.00, 91.81, 90.00 |
Refinement procedure
| Resolution | 46.773 - 2.836 |
| R-factor | 0.2177 |
| Rwork | 0.213 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6H3X along with a Phyre2-generated model of the scFv 1C11 antibody fragment |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.819 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.773 | 3.170 |
| High resolution limit [Å] | 2.836 | 2.836 |
| Rmerge | 0.070 | 0.810 |
| Rmeas | 0.080 | 0.870 |
| Rpim | 0.030 | 0.330 |
| Number of reflections | 16839 | 842 |
| <I/σ(I)> | 15.3 | 2.3 |
| Completeness [%] | 64.2 | 11.4 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 1.000 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 1.0 uL of a 21.7 mg/mL solution of the GPC-purified complex in 20 mM Tris-Cl pH 8.0, 150 mM NaCl was added to 0.75 uL of reservoir solution containing 0.1 M sodium acetate, 8% v/v 2-propanol, 25% w/v PEG 4K |
