6H3S
Schmallenberg Virus Glycoprotein Gc Head/Stalk Domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.919080 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 95.226, 95.226, 204.739 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.613 - 2.023 |
R-factor | 0.2113 |
Rwork | 0.209 |
R-free | 0.24040 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.561 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.930 | 2.280 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.100 | 1.910 |
Rmeas | 0.100 | 1.970 |
Rpim | 0.020 | 0.490 |
Number of reflections | 38793 | 1940 |
<I/σ(I)> | 20.6 | 1.7 |
Completeness [%] | 60.9 | 9.8 |
Redundancy | 17.6 | 15.6 |
CC(1/2) | 1.000 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.5 uL of 12.3 mg/mL EndoH/EndoD-deglycosylated SBV Gc(465-874) in 17.6 mM Tris-Cl pH 8.0, 132 mM NaCl, 0.5 M ammonium sulfate were added to 0.75 uL of reservoir solution containing 0.1 M MES pH6.5, 5%w/v PEG 400, 2 M ammonium sulfate |