6H3P

Crystal structure of the cytoplasmic chorismate mutase from Zea mays

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.8762
Spacegroup name	P 32 2 1
Unit cell lengths	162.598, 162.598, 55.382
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.760 - 2.700
R-factor	0.1868
Rwork	0.184
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3csm
RMSD bond length	0.008
RMSD bond angle	0.987
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.770	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.340	1.628
Number of reflections	23339	2321
<I/σ(I)>	7.26	1.34
Completeness [%]	99.9	99.91
Redundancy	11.4	10.9
CC(1/2)	0.987	0.582

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Sodiumacetate, 24 % PEG 4000