6H3O
Alcohol oxidase from Phanerochaete chrysosporium mutant F101S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-08 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9726 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.074, 203.553, 116.009 |
| Unit cell angles | 90.00, 104.64, 90.00 |
Refinement procedure
| Resolution | 49.190 - 2.500 |
| R-factor | 0.21387 |
| Rwork | 0.210 |
| R-free | 0.28560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h3g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.200 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.657 | |
| Rpim | 0.650 | |
| Number of reflections | 170450 | 8298 |
| <I/σ(I)> | 6.3 | 1.4 |
| Completeness [%] | 98.5 | 96.8 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.979 | 0.485 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 14% (w/v) PEG3350 and 0.2 M potassium acetate |
